GSM7688435: no_nic_T1_R3; Paenarthrobacter nicotinovorans; RNA-Seq - SRA

SRX21286645: GSM7688435: no_nic_T1_R3; Paenarthrobacter nicotinovorans; RNA-Seq
1 OXFORD_NANOPORE (MinION) run: 21,219 spots, 21.4M bases, 19.1Mb downloads

External Id: GSM7688435_r1

Submitted by: BioActive Research Group, Biology, Alexandru Ioan Cuza University of Iasi

Study: Paenarthrobacter nicotinovorans pAO1 strain ATCC 49919 whole genome sequencing and nicotine-induced transcriptome analysis

PRJNA693273 • SRP302373 • All experiments • All runs

show Abstracthide Abstract

Paenarthrobacter nicotinovorans pAO1 is a nicotine degrading microorganism that shows promising applications in converting nicotine-containing waste into useful green chemicals. Its biotechnological applications are nevertheless hampered by the lack of knowledge and tools to perform genetic and metabolic engineering. This BioProject aims to provide the first complete and annotated genome of P. nicotinovorans pAO1 as well as the first nicotine-induced transcriptome.

Sample: no_nic_T1_R3

SAMN36877799 • SRS18536758 • All experiments • All runs

Organism: Paenarthrobacter nicotinovorans

Library:

Name: GSM7688435

Instrument: MinION

Strategy: RNA-Seq

Source: TRANSCRIPTOMIC

Selection: cDNA

Layout: SINGLE

Construction protocol: Native RNA was extracted with the Promega SV Total RNA Isolation System (after 8.C., step 7 were added 8 µl of Proteinase K 20 mg/ml (Promega) and samples were incubated at 37⁰ C for 10 min before proceeding with 4.E, Steps 1–9). Native RNA was polyadenylated using the Lucigen Poly(A) Polymerase Tailing Kit. Library preparation was performed with the Oxford Nanopore Technologies (ONT) Direct RNA sequencing kit (SQK-RNA002) and an adapted protocol for the MinION Flongle flowcells (https://www.protocols.io/view/flongle-directrna-library-preparation-kqdg35847v25/v1 ).

Runs: 1 run, 21,219 spots, 21.4M bases, 19.1Mb

Run	# of Spots	# of Bases	Size	Published
SRR25557717	21,219	21.4M	19.1Mb	2023-09-01

ID:: 28723605

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Sequence Read Archive

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